Tachyplesin-1/TP-1 peptide

Tachyplesin-1/TP-1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KWCFRVCYRGICYRRCR-NH2

H-Lys-Trp-Cys-Phe-Arg-Val-Cys-Tyr-Arg-Gly-Ile-Cys-Tyr-Arg-Arg-Cys-Arg-NH2

17

95.58%

C₉₉H₁₅₁N₃₅O₁₉S₄

2263.73

Synthetic

Powder

Tachyplesin-1 or TP-1 (KWCFRVCYRGICYRRCR-NH2) is a well characterized, structurally and mechanistically, AMP from the hemocyte of horse shoe crab. TP-1 has a broad spectrum of antimicrobial activity against several strains of Gram-negative and Gram-positive bacteria, fungi and viruses. The antimicrobial activity of TP-1 appears to stem from the ability of the peptide to disorganize bacterial cell membranes. NMR studies demonstrated that TP-1 adopts β-hairpin structure in solution and also in lipid micelles and bilayers. The antiparallel β-strands of β-hairpin structure of TP-1 are constrained by the disulfide bridges between residues Cys3–Cys16 and Cys7–Cys12

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Saravanan R, Mohanram H, Joshi M, Domadia PN, Torres J, Ruedl C, Bhattacharjya S. Structure, activity and interactions of the cysteine deleted analog of tachyplesin-1 with lipopolysaccharide micelle: Mechanistic insights into outer-membrane permeabilization and endotoxin neutralization. Biochim Biophys Acta. 2012 Jul;1818(7):1613-24. doi: 10.1016/j.bbamem.2012.03.015. PMID: 22464970.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.