Suc-APA-pNA peptide
Not For Human Use, Lab Use Only.
Cat.#: 309152
Special Price 300.0 USD
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Product Name
Suc-APA-pNA peptide
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Sequence Shortening
Suc-Ala-Pro-Ala-pNA
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Length (aa)
3
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Peptide Purity (HPLC)
98%
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Molecular Formula
C21H27N5O8
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Molecular Weight
477.5
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Source
Synthetic
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Form
Powder
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Description
This is a synthetic colorimetric peptide substrate displaying chymotrypsin-like specificity and is used in the measurement of Kallikreins. It is succinylated at N-terminus and has a nitroanilide tag at the C-terminus.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Clark MR, Aliyar HA, Lee CW, Jay JI, Gupta KM, Watson KM, Stewart RJ, Buckheit RW, Kiser PF. Enzymatic triggered release of an HIV-1 entry inhibitor from prostate specific antigen degradable microparticles. Int J Pharm. 2011 Jul 15;413(1-2):10-18. doi: 10.1016/j.ijpharm.2011.04.004. Epub 2011 Apr 12. PMID: 21511017; PMCID: PMC3109121.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
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• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"