Suc-AEPF-AMC peptide

Suc-AEPF-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Suc-AEPF-AMC

Suc-Ala-Glu-Pro-Phe-AMC

4

96%

C₃₆H₄₁N₅O₁₁

719.73

142997-30-6

99647811

Synthetic

Powder

Suc-AEPF-AMC is a fluorogenic substrate in the assay to measure the peptidyl prolyl isomerase (PPIase) activity of Pin1. AEPF can be utilized by both Pin1 and Par14. The cis to trans isomerization of the Ser - Pro peptide bond proceeds fast, so the assay was performed at 4°C. The reliability of this assay method was confirmed by enzyme kinetic analysis. The identification of Pin1 and Par14 PPIase inhibitors using AEPF as a substrate helped the researchers to investigate the roles of these proteins in cell proliferation and cell cycle progression.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Uchida T, Takamiya M, Takahashi M, Miyashita H, Ikeda H, Terada T, Matsuo Y, Shirouzu M, Yokoyama S, Fujimori F, Hunter T. Pin1 and Par14 peptidyl prolyl isomerase inhibitors block cell proliferation. Chem Biol. 2003 Jan;10(1):15-24. doi: 10.1016/s1074-5521(02)00310-1. PMID: 12573694.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.