Suc-AAPF-AMC peptide

Suc-AAPF-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Suc-AAPF-AMC

Suc-Ala-Ala-Pro-Phe-AMC

4

95.16%

C₃₄H₃₉N₅O₉

661.7

88467-45-2

20833017

Synthetic

Powder

The peptide Suc-Ala-Ala-Pro-Phe-AMC (Suc-AAPF-AMC) is a fluorogenic substrate commonly used in assays to measure the activity of α-chymotrypsin. The Km for Suc-AAPF-AMC with α-chymotrypsin was determined to be 15 μM, and the kcat was 1.5 s⁻¹. These values are comparable to those of other chymotrypsin substrates and were used as a reference to evaluate the performance of the newly synthesized peptide substrate in the ICP-MS assay.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lathia US, Ornatsky O, Baranov V, Nitz M. Development of inductively coupled plasma-mass spectrometry-based protease assays. Anal Biochem. 2010 Mar 1;398(1):93-8. doi: 10.1016/j.ab.2009.11.010. Epub 2009 Nov 11. PMID: 19912984; PMCID: PMC2825755.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.