-
Product Name
Recombinant SSBP protein (His tag)
- Documents
-
Description
Recombinant Human Single-stranded DNA-binding protein, mitochondrial (Mt-SSB) fragment (amino acids 17-148) - a high-quality research tool containing the complete SSB domain for studying mitochondrial DNA replication and maintenance mechanisms. This 132-amino acid fragment retains the essential DNA-binding capabilities of the full-length protein, making it ideal for investigating ssDNA interactions and replisome function.
The full-length Single-stranded DNA-binding protein, mitochondrial (Mt-SSB) is a crucial component in mitochondrial DNA maintenance and replication. Located in the mitochondrial matrix and nucleoid, this protein binds preferentially and cooperatively to pyrimidine-rich single-stranded DNA. Mt-SSB plays a vital role in maintaining mitochondrial DNA copy number and stimulates the activity of key replisome components POLG (DNA polymerase gamma) and TWNK (mitochondrial DNA helicase) during replication. By organizing template DNA and eliminating secondary structures, it promotes ssDNA conformations that facilitate POLG activity and enhances the 5'-3' unwinding function of TWNK. Additionally, evidence suggests potential roles in mitochondrial DNA repair pathways, highlighting its importance in cellular energy production and mitochondrial genome stability.
-
Protein name
Single-stranded DNA-binding protein, mitochondrial
-
Uniprot ID
Q04837
-
Gene Name
SSBP1; SSBP
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
DNA encoding 17-148 aa (Q04837) were fused with 6His tag.
-
Protein Species
Human
-
Molecular weight
Predictes a molecular mass of 16.85 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band.
-
Purity
>89%, by SDS-PAGE with Coomassie Brilliant Blue staining.
-
Activity
Not tested.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"