SpyTag003 peptide

SpyTag003 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-RGVPHIVMVDAYKRYK-OH

H-Arg-Gly-Val-Pro-His-Ile-Val-Met-Val-Asp-Ala-Tyr-Lys-Arg-Tyr-Lys-OH

16

95.86%

C₈₈H₁₄₂N₂₆O₂₁S

1932.29

Synthetic

Powder

SpyTag003 forms a spontaneous amide bond upon binding its genetically encoded partner SpyCatcher (with the latest version SpyCatcher003). SpyTag003 reacts with SpyCatcher003 under a wide range of conditions and the after reaction the product is stable to boiling in SDS. SpyCatcher003 (S49C) has a unique cysteine for precise labeling with dye or precise attachment to surfaces or beads.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Fan C, Cohen AA, Park M, Hung AF, Keeffe JR, Gnanapragasam PNP, Lee YE, Gao H, Kakutani LM, Wu Z, Kleanthous H, Malecek KE, Williams JC, Bjorkman PJ. Neutralizing monoclonal antibodies elicited by mosaic RBD nanoparticles bind conserved sarbecovirus epitopes. Immunity. 2022 Dec 13;55(12):2419-2435.e10. doi: 10.1016/j.immuni.2022.10.019. Epub 2022 Oct 27. PMID: 36370711; PMCID: PMC9606073.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.