SIFamide peptide

SIFamide peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

TRKLPFNGSIF-NH2

H-Thr-Arg-Lys-Leu-Pro-Phe-Asn-Gly-Ser-Ile-Phe-NH2

11

99.46%

C₆₀H₉₄N₁₆O₁₅

1278.5

Synthetic

Powder

SIFamide is a conserved neuropeptide first identified in arthropods, with its amino acid sequence maintained across diverse arthropod lineages, highlighting its evolutionary significance. In Drosophila melanogaster, it is produced by just four neurons in the pars intercerebralis (PI) of the brain, which send widespread projections to key brain regions like the antennal lobes (AL), subesophageal ganglia (SOG), and central complex—areas critical for sensory processing and behavior regulation. Functionally, SIFamide acts as a key integrator in the neuropeptide network controlling feeding: it translates hunger signals into enhanced appetitive behaviors (such as odor- and taste-guided foraging) and increased food intake by modulating the activity of olfactory neurons, particularly sensitizing circuits responsive to appetitive odors. It achieves this by integrating both orexigenic (feeding-stimulating, e.g., hugin-PK) and anorexigenic (feeding-suppressing, e.g., MIP) signals, balancing sensory responsiveness with metabolic state. Beyond feeding, SIFamide also influences other behaviors like courtship and sleep in Drosophila, and its receptor shares evolutionary homology with the vertebrate gonadotropin inhibitory hormone receptor (GnIHR), suggesting conserved roles in prioritizing feeding over other physiological processes (e.g., reproduction) across phyla.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Dircksen H, Neupert S, Predel R, Verleyen P, Huybrechts J, Strauss J, Hauser F, Stafflinger E, Schneider M, Pauwels K, Schoofs L, Grimmelikhuijzen CJ Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones J Proteome Res 2011 Oct 7;10(10):4478-504
• Martelli C, Pech U, Kobbenbring S, Pauls D, Bahl B, Sommer MV, Pooryasin A, Barth J, Arias CWP, Vassiliou C, Luna AJF, Poppinga H, Richter FG, Wegener C, Fiala A, Riemensperger T. SIFamide Translates Hunger Signals into Appetitive and Feeding Behavior in Drosophila. Cell Rep. 2017 Jul 11;20(2):464-478. doi: 10.1016/j.celrep.2017.06.043. PMID: 28700946.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.