Shuchin 4 peptide

Shuchin 4 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KAYSTPRCKGLFRALMCWL-NH2

H-Lys-Ala-Tyr-Ser-Thr-Pro-Arg-Cys-Lys-Gly-Leu-Phe-Arg-Ala-Leu-Met-Cys-Trp-Leu-NH2

19

95.66%

C₁₀₂H₁₆₃N₂₉O₂₂S₃

2243.75

Synthetic

Powder

Shuchin 4 is a peptide that was identified from the skin of the frog Rana shuchinae, China, Asia. Shuchin 4 is a member of a novel family of antimicrobial peptides and has shown antimicrobial activity against various microorganisms, Gram-positive S. aureus, B. cereus, Gram-negative E. coli, B. dysenteriae, P. aeruginosa, and fungus C. albicans (MIC 12-50 ug/ml). Shuchin 4 shows little hemolysis at 200 ug/ml.
The precursor of Shuchin 4 is composed of 64 amino acid residues, including a signal peptide sequence, a spacer peptide fragment, and the mature antimicrobial peptide. The precursor undergoes trypsin-like enzyme processing to release the mature Shuchin 4 peptide. The C-terminus of Shuchin 4 is amidated, and it contains an intramolecular disulfide bridge.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Pei J, Zhao G, Wang B, Wang H. Three novel antimicrobial peptides from the skin of Rana shuchinae. Gene. 2013;521(2):234-7.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.