Recombinant SFPQ protein (His tag)

• DataSheet
• COA (Batch NP456483)
• Material Safety Data Sheets (MSDS)

Recombinant Human SFPQ Fragment (aa 284-592): A high-purity, functional protein fragment encompassing the essential RRM 1 and RRM 2 domains of Splicing Factor, Proline- and Glutamine-Rich (SFPQ/PSF). This product is an ideal tool for studying RNA binding, spliceosome assembly, and transcriptional regulation in a defined, domain-specific context.

SFPQ is a multifunctional nuclear protein involved in critical cellular processes including pre-mRNA splicing, transcriptional regulation, and DNA repair. As a DNA- and RNA-binding protein, it functions as an essential splicing factor required early in spliceosome formation and for the catalytic step of splicing, typically as a heteromer with NONO. The protein specifically binds intronic polypyrimidine tracts and regulates signal-induced alternative splicing. Beyond splicing, SFPQ participates in transcriptional activation and repression, circadian clock regulation, DNA unwinding, and double-strand break repair through non-homologous end joining. It localizes predominantly to nuclear speckles and the nuclear matrix, with some cytoplasmic presence. The provided fragment contains the core RNA recognition motifs (RRM 1 and RRM 2) that mediate nucleic acid binding interactions central to SFPQ's diverse molecular functions.

MSKIKLLRRP GEKTYTQRCR LFVGNLPADI TEDEFKRLFA KYGEPGEVFI NKGKGFGFIK LESRALAEIA KAELDDTPMR
GRQLRVRFAT HAAALSVRNL SPYVSNELLE EAFSQFGPIE RAVVIVDDRG RSTGKGIVEF ASKPAARKAF ERCSEGVFLL
TTTPRPVIVE PLEQLDDEDG LPEKLAQKNP MYQKERETPP RFAQHGTFEY EYSQRWKSLD EMEKQQREQV EKNMKDAKDK
LESEMEDAYH EHQANLLRQD LMRRQEELRR MEELHNQEMQ KRKEMQLRQE EERRRREEEM MIRQREMEEQ MRRQLEHHHH
HH