self-assembling P11-4 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

• QQRFEWEFEQQ polypeptide is a rationally designed self-assembling peptide. This polypeptide is known for its ability to form β-sheet complexes and self-assemble into 3-dimensional fibrillar scaffolds. It can interact with collagen type I fibers, as demonstrated by atomic force microscopy and surface plasmon resonance analyses. The interaction leads to an increase in the width of collagen fibers, which in turn may enhance the resistance of collagen to proteolysis.
• QQRFEWEFEQQ polypeptide also shows promise in dental applications, such as improving the microtensile bonding strength of the bonding interface to artificial caries-affected dentin and decreasing proteolytic activity. It has the potential to contribute to the formation of a more stable hybrid layer with reduced porosity. Overall, this polypeptide offers interesting possibilities for various biomedical and dental applications.

• de Sousa JP, Carvalho RG, Barbosa-Martins LF, Torquato RJS, Mugnol KCU, Nascimento FD, Tersariol ILS, Puppin-Rontani RM. The Self-Assembling Peptide P11-4 Prevents Collagen Proteolysis in Dentin. J Dent Res. 2019 Mar;98(3):347-354. doi: 10.1177/0022034518817351. Epub 2019 Jan 5. PMID: 30612505.
• Gharaei R, Tronci G, Goswami P, Davies RPW, Kirkham J, Russell SJ. Biomimetic peptide enriched nonwoven scaffolds promote calcium phosphate mineralisation. RSC Adv. 2020;10(47):28332-28342. Published 2020 Jul 29. doi:10.1039/d0ra02446e

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.