Sclerosin peptide

Sclerosin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

TPALAVVTTVLPAAAVTTAKSV

Thr-Pro-Ala-Leu-Ala-Val-Val-Thr-Thr-Val-Leu-Pro-Ala-Ala-Ala-Val-Thr-Thr-Ala-Lys-Ser-Val

22

96%

Synthetic

Powder

Warning: this peptide contains non-natural amino acids although they are represented using the closest standard 20 amino acids. Specifically, T1, T8, T17, and T18 are dehydrated into Dhb. P12 is 3,4-dehydrated (Dhp). In total, five residues are dehydrated. Residue K20 is Dab (2,4-diaminobutyric acid). Finally, the acyl chain CH(3)-(CH(2))(6)-CH(OH)-CH(2)-CO is attached to the N-terminus. Thus, the entire sequence is CH(3)-(CH(2))(6)-CH(OH)-CH(2)-CO-Dhb-Pro-Ala-Leu/Ile-Ala-Val-Val-Dhb-Thr-Val-Leu/Ile-Dhp-Ala-Ala-Ala-Val-Dhb-Dhb-Ala-Dab-Ser-Val-OH. Note that residues 4 and 11 can be either L or I. Sclerosin has Antifungal, Antibacterial activity. The source of Sclerosin is Pseudomonas sp. strain DF41.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Berry CL, Brassinga AK, Donald LJ, Fernando WG, Loewen PC, De kievit TR. Chemical and biological characterization of sclerosin, an antifungal lipopeptide. Can J Microbiol. 2012;58(8):1027-34.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.