SAP15 peptide

SAP15 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GKCSTRGRKCCRRKK-OH

H-Gly-Lys-Cys-Ser-Thr-Arg-Gly-Arg-Lys-Cys-Cys-Arg-Arg-Lys-Lys-OH

15

95.68%

C₆₈H₁₃₁N₃₁O₁₈S₃

1767.15

Synthetic

Powder

SAP15 is a cell penetrating peptide corresponding to the last 15 amino acids of the host defense peptide human β-defensin 3, which is involved in adaptive immunity and pro- and anti-inflammatory responses. SAP15 is a histone deacetylase 5 (HDAC5) inhibitor with a dissociation constant of 86 nM in an SPR assay and inhibits lipopolysaccharide (LPS) induced phosphorylation of intracellular HDAC5 and NF-κB p65 in murine macrophages. In vivo, subcutaneous injection of SAP15-loaded sodium hyaluronic acid solution significantly decreases hind paw swelling, joint inflammation, and serum cytokine levels in a collagen induced arthritis (CIA) rat model.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lee et al (2021) Engineered synthetic cell penetrating peptide with intracellular anti-inflammatory bioactivity: An in vitro and in vivo study. J Biomed Mater Res A. 109(10) 2001 PMID: 33818867

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.