S9pep peptide

S9pep peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-ADSPHSSSGMSEVHSPGEHSG-OH

H-Ala-Asp-Ser-Pro-His-Ser-Ser-Ser-Gly-Met-Ser-Glu-Val-His-Ser-Pro-Gly-Glu-His-Ser-Gly-OH

21

95.95%

C₈₂H₁₂₃N₂₇O₃₅S

2079.06

Synthetic

Powder

S9pep is a short sequence of the transcription factor SOX-9, located at the hinge between the high mobility group (HMG) - DNA-binding domain and the SOX-E central conserved domain, that can mimic SOX9 tumour suppressive properties. Like full length SOX9, S9pep inhibits Wnt/β-catenin signaling and the expression of c-Myc, its oncogenic target gene, by relocating β-catenin from the chromatin to the cytosol. S9pep disrupts colorectal cancer cell morphology, reduces the growth of colorectal cancer cells and stem cells, and inhibits tumour development in vivo.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Blache et al (2019) A Short SOX9 Peptide Mimics SOX9 Tumor Suppressor Activity and Is Sufficient to Inhibit Colon Cancer Cell Growth. Mol Cancer Ther. 2019 18(8) 1386 PMID: 31092563

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.