S9pep peptide

S9pep peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-ADSPHSSSGMSEVHSPGEHSG-OH

H-Ala-Asp-Ser-Pro-His-Ser-Ser-Ser-Gly-Met-Ser-Glu-Val-His-Ser-Pro-Gly-Glu-His-Ser-Gly-OH

21

95.95%

C₈₂H₁₂₃N₂₇O₃₅S

2079.06

Synthetic

Powder

S9pep is a short sequence of the transcription factor SOX-9, located at the hinge between the high mobility group (HMG) - DNA-binding domain and the SOX-E central conserved domain, that can mimic SOX9 tumour suppressive properties. Like full length SOX9, S9pep inhibits Wnt/β-catenin signaling and the expression of c-Myc, its oncogenic target gene, by relocating β-catenin from the chromatin to the cytosol. S9pep disrupts colorectal cancer cell morphology, reduces the growth of colorectal cancer cells and stem cells, and inhibits tumour development in vivo.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Blache et al (2019) A Short SOX9 Peptide Mimics SOX9 Tumor Suppressor Activity and Is Sufficient to Inhibit Colon Cancer Cell Growth. Mol Cancer Ther. 2019 18(8) 1386 PMID: 31092563

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.