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  • S-2238 peptide

S-2238 peptide

Not For Human Use, Lab Use Only.

Cat.#: 319786

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Product Information

  • Product Name
    S-2238 peptide
  • Documents

    Batch to batch variation of the purity

  • Sequence
    H-D-Phe-Pip-Arg-pNA
  • Length (aa)
    3
  • Peptide Purity (HPLC)
    95%
  • Molecular Formula
    C29H40N8O7
  • Molecular Weight
    612.68
  • CAS No.
    115388-96-0
  • PubChem CID
    13020985
  • Source
    Synthetic
  • Form
    Powder
  • Description

    S-2238 is a synthetic chromogenic substrate specifically designed for thrombin assays. It consists of the peptide sequence H-D-Phenylalanyl-L-Pipecolyl-Arginine-p-nitroaniline (pNA), which mimics the N-terminal portion of the fibrinogen A alpha chain, the natural substrate of thrombin. Thrombin cleaves the terminal arginine-pNA bond, releasing the chromophore p-nitroaniline, enabling spectrophotometric quantification at 405 nm. The inclusion of phenylalanine and pipecolic acid enhances thrombin specificity and hydrolysis efficiency.

    The substrate demonstrates high solubility and sensitivity to thrombin, allowing shorter reaction times and reduced plasma interference from factors like hyperbilirubinemia or hyperlipidemia. In assays, S-2238 is combined with polybrene to neutralize heparin and optimize thrombin activity measurement. Its stability and reproducibility make it a robust tool for studying serine proteases and their inhibitors in coagulation systems.

  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
  • References
    • Goodnight SH Jr, Schaeffer JL, Sheth K. Measurement of antithrombin III in normal and pathologic states using chromogenic substrate S-2238. Comparison with immunoelectrophoretic and factor Xa inhibition assays. Am J Clin Pathol. 1980 May;73(5):639-47. doi: 10.1093/ajcp/73.5.639. PMID: 7377131.
  • About TFA salt

    Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

    Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

    Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

    In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"