RP-220 peptide

RP-220 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-CIRFVSIDNKKRNIESSEIG-OH

Ac-Cys-Ile-Arg-Phe-Val-Ser-Ile-Asp-Asn-Lys-Lys-Arg-Asn-Ile-Glu-Ser-Ser-Glu-Ile-Gly-OH

20

95.2%

C₁₀₀H₁₆₈N₃₀O₃₃S

2350.64

Synthetic

Powder

RP-220 is a 20 amino acid renalase peptide(aa 220–239: CIRFVSIDNKKRNIESSEIG), which is conserved in all known isoforms but is devoid of any detectable oxidase activity, was equally effective as intact renalase protein at protecting HK-2 cells and WT mice against toxic and ischemic injury. RP-220 and recombinant renalase rapidly activated protein kinase B (AKT), extracellular signal-regulated kinase (ERK), and mitogen activated protein kinase (p38) mitogen activated protein kinases, suggesting that RP-220 is the region of the renalase protein critical for interaction with its cognate receptor. RP-220 also has a pro-survival role for RP-220 following alkaline insult to corneal epithelial cells.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Wang L, Velazquez H, Chang J, Safirstein R, Desir GV. Identification of a receptor for extracellular renalase. PLoS One. 2015;10(4):e0122932. Published 2015 Apr 23. doi:10.1371/journal.pone.0122932
• Potts L, Phillips C, Hwang M, Fulcher S, Choi H. Rescue of human corneal epithelial cells after alkaline insult using renalase derived peptide, RP-220. Int J Ophthalmol. 2019;12(11):1667u20131673. Published 2019 Nov 18. doi:10.18240/ijo.2019.11.01

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.