RP-220 peptide

RP-220 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-CIRFVSIDNKKRNIESSEIG-OH

Ac-Cys-Ile-Arg-Phe-Val-Ser-Ile-Asp-Asn-Lys-Lys-Arg-Asn-Ile-Glu-Ser-Ser-Glu-Ile-Gly-OH

20

95.2%

C₁₀₀H₁₆₈N₃₀O₃₃S

2350.64

Synthetic

Powder

RP-220 is a 20 amino acid renalase peptide(aa 220–239: CIRFVSIDNKKRNIESSEIG), which is conserved in all known isoforms but is devoid of any detectable oxidase activity, was equally effective as intact renalase protein at protecting HK-2 cells and WT mice against toxic and ischemic injury. RP-220 and recombinant renalase rapidly activated protein kinase B (AKT), extracellular signal-regulated kinase (ERK), and mitogen activated protein kinase (p38) mitogen activated protein kinases, suggesting that RP-220 is the region of the renalase protein critical for interaction with its cognate receptor. RP-220 also has a pro-survival role for RP-220 following alkaline insult to corneal epithelial cells.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wang L, Velazquez H, Chang J, Safirstein R, Desir GV. Identification of a receptor for extracellular renalase. PLoS One. 2015;10(4):e0122932. Published 2015 Apr 23. doi:10.1371/journal.pone.0122932
• Potts L, Phillips C, Hwang M, Fulcher S, Choi H. Rescue of human corneal epithelial cells after alkaline insult using renalase derived peptide, RP-220. Int J Ophthalmol. 2019;12(11):1667u20131673. Published 2019 Nov 18. doi:10.18240/ijo.2019.11.01

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.