Ro 25-1553 peptide

Ro 25-1553 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-HSDAVFTENYTKLRKQ{NLE}AAKKYLNDLKKGGT-NH2

Ac-His-Ser-Asp-Ala-Val-Phe-Thr-Glu-Asn-Tyr-Thr-Lys-Leu-Arg-Lys-Gln-Norleucine-Ala-Ala-Lys-Lys-Tyr-Leu-Asn-Asp-Leu-Lys-Lys-Gly-Gly-Thr-NH2

32

96.7%

C₁₆₀H₂₅₈N₄₆O₄₆

3562.1

Synthetic

Powder

Ro 25-1553 is a synthetic 31-amino acid analogue of vasoactive intestinal peptide (VIP). It is characterized by high selectivity for the VPAC₂ receptor subclass. In vitro studies on guinea-pig isolated trachea demonstrate that Ro 25-1553 induces a concentration-dependent and complete relaxation of precontracted tracheal smooth muscle. Its efficacy is comparable to that of the long-acting β₂-adrenoceptor agonist formoterol, though it is 3 to 7 times less potent on a molar basis. The compound exhibits a rapid onset of action and an additive interaction with formoterol. Furthermore, Ro 25-1553 concentration-dependently inhibits nerve-induced contractions in vagus nerve-trachea preparations and retains activity when administered intraluminally, albeit at higher concentrations.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Källström BL, Waldeck B. Bronchodilating properties of the VIP receptor agonist Ro 25-1553 compared to those of formoterol on the guinea-pig isolated trachea. Eur J Pharmacol. 2001 Nov 2;430(2-3):335-40. doi: 10.1016/s0014-2999(01)01299-7. PMID: 11711052.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.