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Product Name
Recombinant RHEB protein (His tag)
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Description
Introducing the recombinant human GTP-binding protein Rheb (RHEB) fragment (residues 1-181), a high-quality reagent designed for studying mTORC1 signaling and cellular growth pathways. This soluble, stable fragment retains key functional domains, making it ideal for structural studies, biochemical assays, and mechanistic investigations of nutrient-sensing regulation.
Rheb is a small GTPase that serves as a critical allosteric activator of the mTORC1 complex, a central nutrient sensor that promotes cellular biomass generation and growth. In response to nutrients, growth factors, or amino acids, Rheb activates MTOR's kinase activity, enhancing phosphorylation of downstream targets like RPS6KB1 and EIF4EBP1. It localizes to lysosomal, Golgi, endoplasmic reticulum, and endomembrane systems via lipid anchoring, with farnesylation required for lysosomal membrane recruitment and mTORC1 activation. Under starvation conditions, Rheb is inactivated by the TSC-TBC complex, highlighting its role in metabolic regulation and cellular homeostasis.
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Protein name
GTP-binding protein Rheb
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Uniprot ID
Q15382
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Gene Name
RHEB; RHEB2
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-181 aa (Q15382) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 21.83 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 22 kDa band.
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Purity
>88%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"