Rb9 peptide

Rb9 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-CARGETARATFAYWGQGC-NH2

H-Cys-Ala-Arg-Gly-Glu-Thr-Ala-Arg-Ala-Thr-Phe-Ala-Tyr-Trp-Gly-Gln-Gly-Cys-NH2

18

95.35%

C₈₃H₁₂₃N₂₇O₂₄S₂

1947.15

Synthetic

Powder

Peptide Rb9 is a cyclic peptide derived from the VHCDR3 of a humanized monoclonal antibody called RebmAb 200. It reacts with an epitope in the sodium-phosphate transporter NaPi2b and displays bio-reactivity independent of mAb specificity. The peptide was synthesized by Peptide 2.0 and modifications included amidation at the C terminus (NH2) and cyclization (C-C, disulfide bridge). It has been shown to have immunomodulatory protective effects in a metastatic melanoma setting and has been tested against syngeneic CT26 colon and Panc02 pancreatic cancer cells grafted subcutaneously in C57Bl/6 mice. It activates murine or human dendritic cells and enhances the immune response against cancer cells. The peptide has been studied for its anti-tumor protective effects against metastatic melanoma and has shown potential as a new anticancer drug.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Machado FC, Girola N, Maia VSC, Bergami-Santos PC, Morais AS, Azevedo RA, Figueiredo CR, Barbuto JAM, Travassos LR. Immunomodulatory Protective Effects of Rb9 Cyclic-Peptide in a Metastatic Melanoma Setting and the Involvement of Dendritic Cells. Front Immunol. 2020 Jan 15;10:3122. doi: 10.3389/fimmu.2019.03122. PMID: 32010152; PMCID: PMC6974543.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.