Pyr-RTKR-AMC peptide

Pyr-RTKR-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Pyr-RTKR-AMC

Pyr-Arg-Thr-Lys-Arg-AMC

5

95.13%

C₃₇H₅₇N₁₃O₉

827.93

155575-02-3

71311916

Synthetic

Powder

The fluorogenic substrate pERTKR-AMC is efficiently cleaved by furin, a mammalian homolog of the yeast Kex2 endoprotease (kcat/Km approx. 2·10⁴ M-¹s-¹). As a calcium-dependent serine protease associated with Golgi membranes, furin is responsible for the secretory processing of precursor proteins at paired basic residues. Also cleaved by proprotein convertase 4. Pyr-RTKR-AMC is an excellent substrate for flavivirin.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lam van TV, Ivanova T, Lindberg I, Böttcher-Friebertshäuser E, Steinmetzer T, Hardes K. Design, synthesis, and characterization of novel fluorogenic substrates of the proprotein convertases furin, PC1/3, PC2, PC5/6, and PC7. Anal Biochem. 2022 Oct 15;655:114836. doi: 10.1016/j.ab.2022.114836. Epub 2022 Aug 11. PMID: 35964735.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.