PUMA BH3 peptide

PUMA BH3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-EEQWAREIGAQLRRMADDLNAQYER-OH

H-Glu-Glu-Gln-Trp-Ala-Arg-Glu-Ile-Gly-Ala-Gln-Leu-Arg-Arg-Met-Ala-Asp-Asp-Leu-Asn-Ala-Gln-Tyr-Glu-Arg-OH

25

95.04%

C₁₂₈H₂₀₂N₄₂O₄₃S

3049.28

Synthetic

Powder

PUMA BH3 is a sequence derived from the BH3 domain of the BH3-only protein PUMA (p53 up-regulated modulator of apoptosis]. PUMA, also known as Bcl-2-binding component 3 (BBC3), is a pro-apoptotic protein and member of the Bcl-2 protein family. Bcl-2 family proteins regulate the mitochondrial apoptosis pathway by controlling mitochondrial outer membrane permeabilization. The BCL2 protein family is divided into three subfamilies: antiapoptotic BCL2 family proteins, including BCL2, BCLxL, and MCL1; proapoptotic multi-domain BCL2 family proteins, including BAK, BAX, and BOK, and proapoptotic BH3-only proteins, including BIM, PUMA, BID, BAD, NOXA, BMF, HRK, and BIK. PUMA acts as a key mediator of cytosolic proapoptotic p53 function by freeing cytosolic p53 from inactive complexes with BCL-xL

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Cartron et al (2004) The First α Helix of Bax Plays a Necessary Role in Its Ligand-Induced Activation by the BH3-Only Proteins Bid and PUMA. Molecular Cell, 16(5) 807 PMID: 15574335
• Fisher et al (2008) Mechanism of apoptosis induction by inhibition of the anti-apoptotic BCL-2 proteins. PNAS 105(51) 20327 PMID: 19074266
• Ye et al (2020) Characterization of an alternative BAK-binding site for BH3 peptides. Nat Commun 11 3301 PMID: 32620849

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.