Pseudhymenochirin-2Pa peptide

Pseudhymenochirin-2Pa peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GIFPIFAKLLGKVIKVASSLISKGRTE-OH

H-Gly-Ile-Phe-Pro-Ile-Phe-Ala-Lys-Leu-Leu-Gly-Lys-Val-Ile-Lys-Val-Ala-Ser-Ser-Leu-Ile-Ser-Lys-Gly-Arg-Thr-Glu-OH

27

95.12%

C₁₃₅H₂₃₀N₃₄O₃₄

2873.46

Synthetic

Powder

Active against S. aureus (MIC 5-10 uM), S. epidermidis (MIC 2.5-10 uM), E. faecalis (MIC 20 um), A. baumannii (10-20 uM), S. maltophilia (10-40 uM), and C. albicans (MIC 80 uM). The peptide is also activ eagainst select cancer cell lines (A549, MDA-231, and HT-29 (LC50 6-11.5 uM) and HUVEC (LC50 68 uM). It is also hemolytic (LC50 6.2 uM)(Mechkarska M et al. 2014). Updated 12/2014. Pseudhymenochirin-2Pa was found in skin secretions, Merlin's clawed frog, Pseudhymenochirus merlini, Africa.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Conlon JM, Prajeep M, Mechkarska M, et al. Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: insights into phylogenetic relationships among the Pipidae. Comp Biochem Physiol Part D Genomics Proteomics. 2013;8(4):352-7.
• Manzo G, Scorciapino MA, Srinivasan D, Attoub S, Mangoni ML, Rinaldi AC, Casu M, Flatt PR, Conlon JM. Conformational Analysis of the Host-Defense Peptides Pseudhymenochirin-1Pb and -2Pa and Design of Analogues with Insulin-Releasing Activities and Reduced Toxicities. J Nat Prod. 2015 Dec 24;78(12):3041-8.
• Mechkarska M, Attoub S, Sulaiman S, Pantic J, Lukic ML, Conlon JM. Anti-cancer, immunoregulatory, and antimicrobial activities of the frog skin host-defense peptides pseudhymenochirin-1Pb and pseudhymenochirin-2Pa. Regul Pept. 2014 Nov;194-195:69-76.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.