PreNAC peptide

PreNAC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GVVHGVATVA-OH

H-Gly-Val-Val-His-Gly-Val-Ala-Thr-Val-Ala-OH

10

95.47%

C₄₀H₆₈N₁₂O₁₂

909.04

Synthetic

Powder

Recent research has shown that a specific protein called CypA plays a key role in the aggregation and toxicity of α-synuclein protein in the body. This protein catalyses the isomerisation of proline 128 in the C-terminal structural domain of α-synuclein, which contributes to its pathogenicity. Additionally, the hydrophobic sequence 47GVVHGVATVA56 forms a second CypA-binding site, which is crucial for the age-dependent aggregation and toxicity of αSyn in the nematode model of Hidradenitis elegans. By deleting this sequence, it is possible to prevent αSyn aggregation and toxicity. Furthermore, this sequence also plays a role in αSyn-mediated vesicle fusion by altering the conformational properties of the protein upon lipid binding. This region could be targeted to prevent αSyn aggregation in disease.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Doherty, C.P.A., Ulamec, S.M., Maya-Martinez, R. et al. A short motif in the N-terminal region of α-synuclein is critical for both aggregation and function. Nat Struct Mol Biol 27,249-259 (2020). https://doi.org/10.1038/s41594-020-0384-x

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.