PPFLMLLKGSTR peptide

PPFLMLLKGSTR peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-PPFLMLLKGSTR-OH

H-Pro-Pro-Phe-Leu-Met-Leu-Leu-Lys-Gly-Ser-Thr-Arg-OH

12

95.32%

C₆₃H₁₀₆N₁₆O₁₅S

1359.67

Synthetic

Powder

PPFLMLLKGSTR is a novel motif identified within the LG3 domain of the human laminin-5 a3 chain. It has been found to be crucial for integrin a3h1 binding and cell adhesion. The PPFLMLLKGSTR peptide within the LG3 domain is capable of supporting integrin a3h1-dependent cell adhesion and spreading. Studies have shown that the Arg residue within the PPFLMLLKGSTR peptide is important for these activities.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Kim JM, Park WH, Min BM. The PPFLMLLKGSTR motif in globular domain 3 of the human laminin-5 alpha3 chain is crucial for integrin alpha3beta1 binding and cell adhesion. Exp Cell Res. 2005 Mar 10;304(1):317-27. doi: 10.1016/j.yexcr.2004.11.009. Epub 2004 Dec 8. PMID: 15707596.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.