POG peptide

POG peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-PPG-OH

H-Pro-Pro-Gly-OH

3

95.36%

C₁₂H₁₉N₃O₄

269.3

Synthetic

Powder

POG polypeptide is a novel biomaterial designed for intervertebral disc regeneration. It is incorporated into a peptide amphiphile molecule (Col - PA) by combining with VVAG sequence and a hydrophobic lauryl tail. The resulting Col - PA, when mixed with E - PA, forms nanofibers with a porous network structure and stable mechanical properties. These nanofibers provide a biocompatible environment for cells such as rat mesenchymal stem cells (rMSCs), supporting their growth and proliferation.
In vitro experiments demonstrate that POG - containing nanofibers can induce chondrogenic differentiation of MSCs, as evidenced by increased glycosaminoglycan deposition and Sox9 gene expression. In vivo studies using a rabbit needle puncture model of intervertebral disc degeneration show that the Col - PA/E - PA nanofiber scaffold promotes tissue regeneration, with increased deposition of glycosaminoglycans and collagen and reduced degeneration scores compared to control groups. Overall, POG polypeptide - based scaffolds offer a promising solution for the treatment of intervertebral disc degeneration and related spinal disorders.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Fiala T, Barros EP, Ebert MO, Ruijsenaars E, Riniker S, Wennemers H. Frame Shifts Affect the Stability of Collagen Triple Helices. J Am Chem Soc. 2022 Oct 12;144(40):18642-18649. doi: 10.1021/jacs.2c08727. Epub 2022 Sep 30. PMID: 36179150.
• Uysal O, Arslan E, Gulseren G, Kilinc MC, Dogan I, Ozalp H, Caglar YS, Guler MO, Tekinay AB. Collagen Peptide Presenting Nanofibrous Scaffold for Intervertebral Disc Regeneration. ACS Appl Bio Mater. 2019 Apr 15;2(4):1686-1695. doi: 10.1021/acsabm.9b00062. Epub 2019 Mar 26. PMID: 35026903.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.