PKI (14-22) amide, myristoylated peptide

PKI (14-22) amide, myristoylated peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Myrisitic Acid-GRTGRRNAI-NH2

Myrisitic Acid-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-NH2

9

95.76%

C₅₃H₁₀₀N₂₀O₁₂

1209.48

Synthetic

Powder

PKI (14 - 22) amide, myristoylated, is a cell-permeable version of the protein kinase inhibitor (PKI) PKI (14-22) amide. When non-myristoylated, PKI (14-22) amide inhibits protein kinase A with a Ki of 36 nM and also inhibits cell growth and induces apoptosis in human pancreatic cancer cells (PANC-1). Myristoylated peptides as a class may exhibit off-target activation of the funny current If in sinoatrial myocytes.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Glass et al (1989) Primary structural determinants essential for potent inhibition of cAMP-dependent protein kinase by inhibitory peptides corresponding to the active portion of the heat-stable inhibitory protein. J.Biol.Chem. 264 8802 PMID: 2722799
• Farrow et al (2003) Inhibition of pancreatic cancer cell growth and induction of apoptosis with novel therapies directed against protein kinase A. Surgery 134 197 PMID: 12947318
• Liao et al (2011) Myristoylated peptides potentiate the funny current I(f) in sinoatrial myocytes. Channels 5(2) 115 doi:10.4161/chan.5.2.14195

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.