Pis2 peptide

Pis2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FLHHIVGLIHHGLSLFGDRAD

Phe-Leu-His-His-Ile-Val-Gly-Leu-Ile-His-His-Gly-Leu-Ser-Leu-Phe-Gly-Asp-Arg-Ala-Asp

21

97.7%

Synthetic

Powder

Active against Gram-negative bacteria Y. ruckeric NCIMB 2196 and P. immobilisc NCIMB 308 (MIC 2.5-5 uM). A recent study synthesized a peptide variant GAD-2 without the last two C-terminal residues (McDonald M et al., 2015). GAD-2 is much more active at slightly acidic pH. Both Gad-1 and Gad-2 demonstrated concentration-dependent cytotoxicity against LLC cells, HEY ovarian cancer cells, and PC3 prostate cancer cells. Helical in SDS. Structural heterogeneity is proposed. Updated 4/2015. Pis2 has Antiparasitic activity. The source of Pis2 is Atlantic cod, Gadus morhua L..

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ruangsri J, Salger SA, Caipang CM, Kiron V, Fernandes JM. Differential expression and biological activity of two piscidin paralogues and a novel splice variant in Atlantic cod (Gadus morhua L.). Fish Shellfish Immunol. 2012;32(3):396-406.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.