Pis1 peptide

Pis1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FIHHIIGWISHGVRAIHRAIHG

Phe-Ile-His-His-Ile-Ile-Gly-Trp-Ile-Ser-His-Gly-Val-Arg-Ala-Ile-His-Arg-Ala-Ile-His-Gly

22

97.1%

Synthetic

Powder

It is likely that the last Glycine can be converted to C-terminal amidation (e.g., GAD-1) (McDonald M et al., 2015). These authors found that both Gad-1 and Gad-2 demonstrated concentration-dependent cytotoxicity against LLC cells, HEY ovarian cancer cells, and PC3 prostate cancer cells. Ruangsri et al. found it active against Gram-positive bacteria M. luteus (M. lysodeikticus) ATCC 4698, P. citreus NCIMB 1493, C. glutamicum ATCC 13032, S. aureus ATCC 9144 (MIC , 0.04-5 uM) and Gram-negative bacteria E. coli ATCC 25922, V. anguillarum NCIMB 2133, V. anguillarum NCIMB 2129, Y. ruckeric NCIMB 2196, Y. ruckeric VI-3629, and P. immobilisc NCIMB 308 (MIC 0.3-10 uM). The C-terminal 17 residues are helical in SDS. Updated 4/2015. Pis1 has Antibacterial activity. The source of Pis1 is Atlantic cod, Gadus morhua L..

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ruangsri J, Salger SA, Caipang CM, Kiron V, Fernandes JM. Differential expression and biological activity of two piscidin paralogues and a novel splice variant in Atlantic cod (Gadus morhua L.). Fish Shellfish Immunol. 2012;32(3):396-406.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.