Cart 2

Recombinant PIM1 protein (His tag)

Cat.#: 542109

Size:

Special Price 383.1 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Recombinant PIM1 protein (His tag)
  • Documents
  • Description

    Recombinant Human Serine/threonine-protein kinase pim-1 (EC 2.7.11.1) fragment (residues 31-313) is a high-purity protein encompassing the complete protein kinase domain (residues 38-290). This fragment serves as an essential tool for investigating kinase activity, oncogenic signaling pathways, and drug discovery applications related to cell survival and proliferation mechanisms.

    Serine/threonine-protein kinase pim-1, encoded by the PIM1 gene, functions as a proto-oncogene that promotes cell survival and proliferation through multiple mechanisms. It regulates MYC transcriptional activity, controls cell cycle progression at G1-S and G2-M transitions by phosphorylating CDC25A and CDC25C, and inhibits apoptosis by phosphorylating proapoptotic proteins including BAD, MAP3K5, and FOXO3. The protein also modulates mTORC1 signaling through DEPDC5 phosphorylation and affects drug resistance via ABCG2 transporter activation. In its full-length form, pim-1 localizes to both the cytoplasm and nucleus (isoform 1

  • Protein name
    Serine/threonine-protein kinase pim-1
  • Uniprot ID
    P11309
  • Gene Name
    PIM1
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    DNA encoding 31-313 aa (P11309) were fused with C-terminal 6His tag.
  • Sequence 
    MSKIKKEPLE SQYQVGPLLG SGGFGSVYSG IRVSDNLPVA IKHVEKDRIS DWGELPNGTR VPMEVVLLKK VSSGFSGVIR
LLDWFERPDS FVLILERPEP VQDLFDFITE RGALQEELAR SFFWQVLEAV RHCHNCGVLH RDIKDENILI DLNRGELKLI
DFGSGALLKD TVYTDFDGTR VYSPPEWIRY HRYHGRSAAV WSLGILLYDM VCGDIPFEHD EEIIRGQVFF RQRVSSECQH
LIRWCLALRP SDRPTFEEIQ NHPWMQDVLL PQETAEIHLH SLSPGPSKLE HHHHHH
  • Protein Species
    Human
  • Molecular weight
    Predictes a molecular mass of 34.13 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 36 kDa band.
  • Purity
    >90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
  • Activity
    Not tested.
  • Validations

    Recombinant PIM1 protein (His tag) SDS-PAGE

    Recombinant PIM1 protein (His tag) SDS-PAGE

    M: Marker; Lane 1: PIM1; Lane 2: BSA

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"