Phylloseptin-L1 peptide

Phylloseptin-L1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

LLGMIPLAISAISALSKL

Leu-Leu-Gly-Met-Ile-Pro-Leu-Ala-Ile-Ser-Ala-Ile-Ser-Ala-Leu-Ser-Lys-Leu

18

97.9%

Synthetic

Powder

Phylloseptin-L1 was active against S. aureus (MIC=8 uM). This peptide also inhibited growth of B. dendrobatidis zoospores at concentrations above 25 uM with complete inhibition at 100 uM. Phylloseptin-L1 was approximately equipotent against both HepG2 cells (LC50=35 uM) and erythrocytes (LC50=40 uM). Xi X et al (2013) identified the same peptide in a different frog from Central America. Their peptide was active against S. aureus (MIC 8 mg/L) and C. albicans (MIC 32 mg/L). Hemolytic 80% at 128 mg/L. Found in multiple species. Updated 3/15/2013 GW. Phylloseptin-L1 has Antibacterial, Antifungal activity. Phylloseptin-L1 was found in Hylomantis lemur (Hylidae: Phyllomedusinae, South America; also skin secretion, red-eyed leaf frog, Agalychnis callidryas, Central America.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Conlon JM, Woodhams DC, Raza H, et al. Peptides with differential cytolytic activity from skin secretions of the lemur leaf frog Hylomantis lemur (Hylidae: Phyllomedusinae). Toxicon. 2007;50(4):498-506.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.