Peptide AN5-1

Peptide AN5-1

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

YSKSLPLSVLNP

Tyr-Ser-Lys-Ser-Leu-Pro-Leu-Ser-Val-Leu-Asn-Pro

12

98.2%

Synthetic

Powder

bactericidal, inducing cell lysis in E. coli ATCC 29522 and S. aureus, and non-cell lysis in both S. marcescens and B. cereus ATCC 14579. MIC 4-8 ug/ml against S. enteritidis ATCC 13076 and E. coli; 16-32 ug/ml against Enterobacter spp.,S. marcescens, L. plantarum ATCC 8014, B. subtilis; 64 ug/ml against B. cereus ATCC 14579 and S. aureus; and 128 ug/ml against B. anthracis. This peptide can disrupt bacterial membranes and bind to DNA (Yi T et al., 2014). Updated 10/2014. Peptide AN5-1 has Antibacterial activity. The source of Peptide AN5-1 is Paenibacillus alvei AN5.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Alkotaini B, Anuar N, Kadhum AA, Sani AA. Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5. J Ind Microbiol Biotechnol. 2013;40(6):571-9.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.