Pep8 (Pp3c14_22870V3) peptide

Pep8 (Pp3c14_22870V3) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-INIINAPLQGFKIA-OH

H-Ile-Asn-Ile-Ile-Asn-Ala-Pro-Leu-Gln-Gly-Phe-Lys-Ile-Ala-OH

14

95.2%

C₇₁H₁₁₈N₁₈O₁₈

1511.8

Synthetic

Powder

Using mass-spectrometry, in vitro experiments and bioinformatics analysis, we found that methyl jasmonate acid induces significant changes in the peptide pools and that some of the resulting peptides possess antimicrobial and regulatory activities. Moreover, our study provides a list of peptides for further study of potential plant cryptides. We found that the minimum inhibitory concentration (MIC) for two peptides, LVQIGTKIVGVGRNYAAH (pep1, a fragment of fumarylacetoacetate hydrolase domain-containing protein) from the cell and INIINAPLQGFKIA (pep8, a fragment of the predicted protein) from the cell secretome were 64 and 16 μg/mL, respectively.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Fesenko I, Azarkina R, Kirov I, et al. Phytohormone treatment induces generation of cryptic peptides with antimicrobial activity in the Moss Physcomitrella patens. BMC Plant Biol. 2019;19(1):9. Published 2019 Jan 7. doi:10.1186/s12870-018-1611-z

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.