Pep-RTYM peptide

Pep-RTYM peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-DHVTPDIAYNPRTMY-OH

H-Asp-His-Val-Thr-Pro-Asp-Ile-Ala-Tyr-Asn-Pro-Arg-Thr-Met-Tyr-OH

15

95.2%

C₇₉H₁₁₇N₂₁O₂₅S

1792.95

Synthetic

Powder

Pep-RTYM is a synthetic bioactive peptide derived from <i>Acacia catechu</i>. Pep-RTYM inhibited DENV infection with a half-maximal inhibition concentration value of 7.9 uM. Time-of-addition study demonstrated that Pep-RTYM interacted with DENV particles and inhibited cellular entry. Pep-RTYM at 50 uM significantly reduced DENV production in Vero-kidney epithelial cells about 1000-fold, but it could decrease the virus production in Huh7 hepatocyte cells approximately 40-fold. Binding of Pep-RTYM to DENV particles may prevent virus interaction with cellular receptor and subsequent virus entry. Pep-RTYM interacts with DENV particles and inhibited cell entry, and inhibited DENV replication and production in a dose-dependent manner. This finding suggests a potential role of Pep-RTYM in the development of a novel anti-DENV drug.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Panya, A.; Sawasdee, N.; Songprakhon, P.; Tragoolpua, Y.; Rotarayanont, S.; Choowongkomon, K.; Yenchitsomanus, P.-T. A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry. Viruses 2020, 12, 1267.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.