Pep-20 peptide

Pep-20 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-AWSATWSNYWRH-OH

H-Ala-Trp-Ser-Ala-Thr-Trp-Ser-Asn-Tyr-Trp-Arg-His-OH

12

99.42%

C₇₄H₉₃N₂₁O₁₈

1564.73

Synthetic

Powder

Pep-20 peptide (AWSATWSNYWRH) was able to block CD47/SIRPα pathway by binding to CD47. An in vitro study confirmed the ability of Pep-20 to enhance macrophage phagocytic activity against a range of murine and human cancer cell lines as well as promoting macrophage-mediated development of antitumor CD8+ T cells. Antitumor activity of Pep-20 was also observed in a CT26 tumor mouse model, where the Pep-20 treated group showed tumor suppression and increased overall survival.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wang H, Sun Y, Zhou X, Chen C, Jiao L, Li W, et al.. CD47/SIRPα Blocking Peptide Identification and Synergistic Effect With Irradiation for Cancer Immunotherapy. J Immunother Cancer (2020) 8(2):e000905. doi: 10.1136/jitc-2020-000905

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.