Penetratin peptide

Penetratin peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RQIKIWFQNRRMKWKK-OH

H-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-OH

16

97.21%

C₁₀₄H₁₆₈N₃₄O₂₀S

2246.76

Synthetic

Powder

Penetratin, a 16-mer Drosophila Antennapedia derived peptide, like TAT, crosses the biological membranes and is transported to the nucleus of the cells. The interaction between penetratin (P16; RQIKIWFQNRRMKWKK) and phospholipid bicelles mimicking biological membranes was studied by NMR spectroscopy. The secondary structure and positioning studies confirmed the two tryptophan residues (48W and 56W) as the most important amino acids required for cellular internalization . The P16 or its shortened variant P7 (RRMKWKK) has been utilized to promote cellular uptake of impermeable targeting peptides such as G7-18NATE. Antitumor efficacy and translocation across the BBB of liposomes or liposome encapsulated drugs (doxorubicin and erlotinib) were evaluated using the in vitro BBB and intracranial glioblastoma xenograft models. Dual conjugation of penetratin and transferrin to liposomes (Tf-Pen-Lipo) significantly enhanced the BBB translocation. In addition, doxorubicin/erlotinib containing Tf-Pen-Liposomes very significantly accumulated in tumors, inhibited tumor growth, and prolonged survival rate when compared to the free drugs.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Pietersz GA. et al. Vaccine. 19:1397-1405 (2001)
• Christiaens B. et al. Eur J Biochem. 27(16):1187-1197 (2004).

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.