PD-L1Pep-2 peptide

PD-L1Pep-2 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-CVRARTR-OH

H-Cys-Val-Arg-Ala-Arg-Thr-Arg-OH

7

95.73%

C₃₃H₆₄N₁₆O₉S

861.02

Synthetic

Powder

PD-L1Pep-2 was identified from a phage-displayed library for peptides that selectively bind to PD-L1-overexpressing cells. PD-L1Pep-2 has a binding affinity of 281 nM, and preferentially binds to high PD-L1-expressing cells over low PD-L1-expressing cells, binding further enhanced by interferon-γ, an inducer of PD-L1 expression. In animal models, intravenously injected PD-L1Pep-2 efficiently targets tumour tissues, inhibits tumour growth, and increases the CD8+/FoxP3+ ratio in mice. PD-L1Pep-2 in combination with doxorubicin inhibits tumour growth more efficiently than doxorubicin alone. PD-L1Pep-2 blockade of PD-L1 reinvigorates T-cell activity and inhibits tumour growth by enhancing anti-tumour immunity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Gurung et al (2020) Phage display-identified PD-L1-binding peptides reinvigorate T-cell activity and inhibit tumor progression. 247 119984 PMID: 32278214
• Park et al (2022) Abstract 3422: Combined treatment with interleukin-4 receptor-targeted cytotoxic peptide and PD-L1-blocking peptide efficiently induces immunogenic cell death of tumor cells and activates T-cell activity. Cancer Res 82 (12 Supplement) 3422. https://doi.org/10.1158/1538-7445.AM2022-3422

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.