PapRIV peptide

PapRIV peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SDLPFEH-OH

H-Ser-Asp-Leu-Pro-Phe-Glu-His-OH

7

97.44%

C₃₈H₅₃N₉O₁₃

843.87

Synthetic

Powder

PapRIV is a quorum sensing heptapeptide originating from the Bacillus cereus group, which comprises a number of highly related species. PapRIV possesses in vitro pro-inflammatory effects on microglial BV-2 cell lines and is able to cross the gastro-intestinal tract and the blood–brain barrier. PapRIV shows very high brain influx, suggesting a role in gut-brain signalling.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Grenha et al (2013) Structural basis for the activation mechanism of the PlcR virulence regulator by the quorum-sensing signal peptide PapR. Proc Natl Acad Sci U S A. 110(3) 1047 PMID: 23277548
• Janssens et al (2021) PapRIV, a BV-2 microglial cell activating quorum sensing peptide. Sci Rep. 11(1) 10723 PMID: 34021199

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.