Pancragen peptide

Pancragen peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KEDW-OH

H-Lys-Glu-Asp-Trp-OH

4

95.62%

C₂₆H₃₆N₆O₉

576.6

Synthetic

Powder

Pancragen is a tetrapeptide composed of the amino acid sequence Lys-Glu-Asp-Trp (KEDW). It is a synthetic analog derived from a peptide originally isolated from bovine pancreatic cells and has been identified as a potential peptide bioregulator with implications for pancreatic function and age-associated metabolic processes. Aimed at supporting pancreatic function, pancragen can interact with histones and DNA, potentially improving glucose metabolism and insulin production. It may also aid in tissue repair within the pancreas.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Fedoreyeva LI, Smirnova TA, Kolomijtseva GY, Khavinson VKh, Vanyushin BF. Interaction of short peptides with FITC-labeled wheat histones and their complexes with deoxyribooligonucleotides. Biochemistry (Mosc). 2013 Feb;78(2):166-75. doi: 10.1134/S0006297913020053. PMID: 23581987.
• Goncharova ND, Ivanova LG, Oganian TÉ, Vengerin AA, Khavinson VKh. [Impact of tetrapeptide pancragen on endocrine function of the pancreas in old monkeys]. Adv Gerontol. 2014;27(4):662-7. Russian. PMID: 25946840.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.