PAM-5 peptide

PAM-5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KWKWRPLKRKLVLRM-OH

H-Lys-Trp-Lys-Trp-Arg-Pro-Leu-Lys-Arg-Lys-Leu-Val-Leu-Arg-Met-OH

15

95.96%

C₉₇H₁₆₄N₃₀O₁₆S

2038.59

Synthetic

Powder

PAM-5 is a potent antibacterial peptide that was developed through phage display selection and computer-assisted modification. It has enhanced antibacterial features, as demonstrated by its ability to cause membrane disruption and permeabilization of bacteria, bind bacterial DNA, and kill the bacteria rapidly in 10 minutes based on time-kill assay results. PAM-5 also exhibits stability in human plasma via ex vivo assays while being non-cytotoxic towards Vero cells at all concentrations tested for the antibacterial assays.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Yuen HL, Chan SY, Ding YE, Lim S, Tan GC, Kho CL. Development of a Novel Antibacterial Peptide, PAM-5, via Combination of Phage Display Selection and Computer-Assisted Modification. Biomolecules. 2023; 13(3):466. https://doi.org/10.3390/biom13030466

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.