PACAP-38 (6-38) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

PACAP-38 (6-38) is a truncated form of pituitary adenylate cyclase-activating polypeptide (PACAP), specifically lacking the first five N-terminal amino acids. This peptide functions as a selective antagonist for the PAC1 receptor, one of the primary receptors for PACAP. PACAP and its receptors, including PAC1, are widely expressed in the nervous system and peripheral tissues, where they regulate diverse physiological processes such as neurotransmission and smooth muscle activity.

In studies involving NGF-overexpressing (NGF-OE) mice, intravesical administration of PACAP-38 (6-38) at 300 nM significantly modulated bladder function by increasing intercontraction intervals and void volumes while reducing baseline bladder pressure and non-voiding contractions. The peptide also decreased referred pelvic sensitivity in these mice, highlighting its role in PACAP/PAC1 signaling pathways associated with visceral sensation and smooth muscle regulation. No effects were observed in wild-type mice, indicating specificity in pathological conditions involving NGF overexpression.

• P.Robberecht et al., Eur. J. Biochem., 207, 239 (1992)
• P.Robberecht et al., Mol. Pharmacol., 42, 347 (1992)
• F.Ohno et al., Regul. Peptides, 126, 115 (2005)
• Girard BM, Malley SE, Mathews MM, May V, Vizzard MA. Intravesical PAC1 Receptor Antagonist, PACAP(6-38), Reduces Urinary Bladder Frequency and Pelvic Sensitivity in NGF-OE Mice. J Mol Neurosci. 2016 Jun;59(2):290-9. doi: 10.1007/s12031-016-0764-1. Epub 2016 May 4. PMID: 27146136; PMCID: PMC5252828.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.