P1 peptide

P1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FLYTLLREV

Phe-Leu-Tyr-Thr-Leu-Leu-Arg-Glu-Val

9

95%

C₅₆H₈₈N₁₂O₁₄

1153.39

Synthetic

Powder

P1 (FLYTLLREV) is a 9-mer HLA-A02:01-restricted cytotoxic T lymphocyte (CTL) epitope derived from the Six-Transmembrane Epithelial Antigen of the Prostate 1 (STEAP1), mapping to the 86–94 amino acid region of the STEAP1 protein (UniProt ID: Q9UHE8). This peptide exhibits a high predicted binding affinity to HLA-A02:01 (percentile rank 0.03, binding score 0.94) and robust structural compatibility with the MHC class I binding groove, as validated by molecular docking with a binding energy of −246.57. Characterized by an α-helical secondary structure in aqueous physiological conditions, FLYTLLREV elicits potent antigen-specific IFN-γ secretion and efficient activation of polyfunctional CD8+ T cells with co-expression of IFN-γ and TNF-α, demonstrating strong immunogenicity in HLA-A*02:01 transgenic murine models. It also shows high sequence conservation across homologs and favorable proteasomal processing potential, supporting its natural presentation via the endogenous MHC class I antigen presentation pathway.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Yueting Huang, Yang Yang, Yanni Xie, Yue Shu, Siqi Yang, Xuezhi Long, Zhipeng Wen, Xiaolu Duan, Li Fu, Di Gu & Tuxiong Huang (2026) Preclinical Assessment of HLAA*02:01-Restricted PSMA and STEAP1 Epitopes for Peptide-Based Immunotherapy in Prostate Cancer, Drug Design, Development and Therapy, , 576346, DOI: 10.2147/DDDT.S576346

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.