-
Product Name
Recombinant OSGEP protein (His tag)
- Documents
-
Description
Recombinant Human tRNA N6-adenosine threonylcarbamoyltransferase (OSGEP) Fragment (1-335) is a high-purity, soluble protein fragment ideal for structural biology and enzymatic studies. This fragment encompasses the full-length protein (residues 1-335) and is designed for investigating tRNA modification mechanisms and the catalytic role of OSGEP in the EKC/KEOPS complex.
The full-length tRNA N6-adenosine threonylcarbamoyltransferase (OSGEP) is a key component of the EKC/KEOPS complex, essential for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that decode codons starting with adenine. This enzymatic activity involves the transfer of the threonylcarbamoyl moiety from threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, with OSGEP playing a direct catalytic role, supported by other proteins in the complex. The protein localizes to both the cytoplasm and nucleus, underscoring its importance in fundamental cellular processes related to tRNA maturation and translation fidelity.
-
Protein name
tRNA N6-adenosine threonylcarbamoyltransferase
-
Uniprot ID
Q9NPF4
-
Gene Name
OSGEP; GCPL1
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
DNA encoding 1-335 aa (Q9NPF4) were fused with 6His tag.
-
Protein Species
Human
-
Molecular weight
Predictes a molecular mass of 38.08 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 38 kDa band.
-
Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
-
Activity
Not tested.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"