OH-CATH30 peptide

OH-CATH30 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-KFFKKLKNSVKKRAKKFFKKPRVIGVSIPF-OH

H-Lys-Phe-Phe-Lys-Lys-Leu-Lys-Asn-Ser-Val-Lys-Lys-Arg-Ala-Lys-Lys-Phe-Phe-Lys-Lys-Pro-Arg-Val-Ile-Gly-Val-Ser-Ile-Pro-Phe-OH

30

95.01%

C₁₇₅H₂₈₉N₄₇O₃₄

3595.45

Synthetic

Powder

OH-CATH30 peptide is a 34-amino acid peptide derived from the king cobra cathelicidin. It has been found to exhibit strong antibacterial activity and weak hemolytic activity. It has potential broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria, including multi-drug-resistant strains such as Acinetobacter spp. and methicillin-resistant Staphylococcus aureus (MRSA). OH-CATH30 and its analogs, including D-OH-CATH30, have shown higher efficacy against clinical isolates compared to routinely used antibiotics. These peptides have also demonstrated low cytotoxicity to eukaryotic cells and the ability to stimulate the immune system to protect against infections. OH-CATH30 is considered a promising candidate drug for the treatment of bacterial infections that are resistant to commonly used antimicrobial agents.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Zhao F, Lan XQ, Du Y, Chen PY, Zhao J, Zhao F, Lee WH, Zhang Y. King cobra peptide OH-CATH30 as a potential candidate drug through clinic drug-resistant isolates. Zool Res. 2018 Mar 18;39(2):87-96. doi: 10.24272/j.issn.2095-8137.2018.025. PMID: 29515090; PMCID: PMC5885386.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.