Ocellatin 4 peptide

Ocellatin 4 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GLLDFVTGVGKDIFAQLIKQI-NH2

H-Gly-Leu-Leu-Asp-Phe-Val-Thr-Gly-Val-Gly-Lys-Asp-Ile-Phe-Ala-Gln-Leu-Ile-Lys-Gln-Ile-NH2

21

95.52%

C₁₀₇H₁₇₆N₂₆O₂₈

2274.69

Synthetic

Powder

Ocellatin 4 is a cytolytic peptide found in the skin secretion of the frog Leptodactylus ocellatus, Brazil, South America. It exhibits cytolytic activity against human erythrocytes, with an HC50 value of 14.3 mM.
Ocellatin 4 also shows antibacterial activity against E. coli and S. aureus, with MIC values of 64 mM. The peptide interacts preferentially with blood-cell membranes and has a weak charge at pH 7.0.
Ocellatin 4 has a neutral charge at pH 7.0, which may favor its interaction with zwitterionic phospholipids in mammalian plasma membranes.
Ocellatin 4 may self-associate in aqueous solution, which can enhance its hemolytic activity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Nascimento A, Chapeaurouge A, Perales J, et al. Purification, characterization and homology analysis of ocellatin 4, a cytolytic peptide from the skin secretion of the frog Leptodactylus ocellatus. Toxicon. 2007;50(8):1095-104.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.