NT-1 peptide

NT-1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-TLWPFDLWLKTR-OH

H-Thr-Leu-Trp-Pro-Phe-Asp-Leu-Trp-Leu-Lys-Thr-Arg-OH

12

95.86%

C₇₈H₁₁₄N₁₈O₁₇

1575.84

Synthetic

Powder

NT-1 at extremely low concentration (3 nM) in solutions could be efficiently captured by immobilized sTNP(standard tyrosine-nitrated peptide) as determined by pull-down and subsequent MALDI-TOF MS analysis. Surface plasmon resonance (SPR) measurement confirmed that NT-1 possesseed a good selectivity, showing more than 100-fold higher binding affinity with TNP than its non-nitrated counterpart. Moreover, NT-1 could efficiently capture various types of TNPs in solutions even in the presence of 1000-fold excessive amount of trypsinized BSA fragments. Most importantly, NT-1 showed superiority to commercially used nitrotyrosine antibody as the former captured more TNPs, with less sequence preference.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Cheng Y, Qi J, Liu K, Zhou C, Wang W, Zuo Y, Zhang P, Liang S, Wang Y, Chen P, Tang C, Liu Z. Characterization of a novel affinity binding ligand for tyrosine nitrated peptides from a phage-displayed peptide library. Talanta. 2022 May 1;241:123225. doi: 10.1016/j.talanta.2022.123225. Epub 2022 Jan 13. PMID: 35066280.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.