Nonaarginine/(Arg)9 peptide

Nonaarginine/(Arg)9 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

RRRRRRRRR

H-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-OH

9

96.83%

C₅₄H₁₁₀N₃₆O₁₀

1423.67

2283335-13-5

Synthetic

Powder

Nonaarginine/(Arg)9 is a cell-penetrating peptide comprising 9 arginine residues capable of traversing plasma membranes. It has been shown that Arg9 destabilizes and disrupts the phospholipid bilayer allowing flow of ions across the membrane. (Arg)9 peptide plays an important role in efficient cellular uptake. The guanidinium group is a critical structural determinant for tight and rapid interactions with cell membranes. The cationic guanidinium group can form electrostatic interactions with anionic cell membrane components such as phospholipids and sulfated proteoglycans. This interaction can trigger the activation of specific intracellular signaling and cell internalization via various pathways. (Arg)9 peptide delivers a variety of functionally active cargos such as peptides, small interfering RNA, oligonucleotides, plasmid DNA, and liposomes into mammalian cells and plant cells with high translocation efficiency. (Arg)9 peptide can also form chemical linkages with the cargo and act as a carrier peptide.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• J.P.Richard et al., J. Biol. Chem., 278, 585 (2003)
• S.M.Fuchs and R.T.Raines, Biochemistry, 43, 2438 (2004)
• R.Fischer et al., ChemBioChem, 7, 1428 (2006)

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.