Neurite Outgrowth-promoting Site peptide

Neurite Outgrowth-promoting Site peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-VFDNFVLK-OH

H-Val-Phe-Asp-Asn-Phe-Val-Leu-Lys-OH

8

95.5%

C₄₈H₇₂N₁₀O₁₂

981.14

Synthetic

Powder

FPGERGVEGPGP polypeptide is an eight-amino acid sequence within the alternatively spliced FN - III repeat D of human tenascin - C. It has been identified as crucial for neurite outgrowth promotion. This peptide is active by itself and also functions in the context of the large tenascin - C splice variant. The biological activity of FPGERGVEGPGP depends on specific amino acids within the sequence.
It can overcome the inhibitory effect of chondroitin sulfate proteoglycans (CSPGs) on neurite outgrowth in vitro, which is significant as CSPGs are upregulated in glial scars and impede axonal regrowth. In neural tissue engineering applications, it has the potential to enhance neuronal regeneration and differentiation. For example, in studies with PC12 cells, related peptide nanofibers have been shown to increase the length and number of neurites in 2D and 3D cultures, and promote the expression of neural markers such as β - III tubulin and synaptophysin, suggesting its possible role in the development of therapies for neurological conditions.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Sever M , Gunay G , Guler MO , Tekinay AB . Tenascin-C derived signaling induces neuronal differentiation in a three-dimensional peptide nanofiber gel. Biomater Sci. 2018 Jun 25;6(7):1859-1868. doi: 10.1039/c7bm00850c. PMID: 29799029.
• Mercado ML, Nur-e-Kamal A, Liu HY, Gross SR, Movahed R, Meiners S. Neurite outgrowth by the alternatively spliced region of human tenascin-C is mediated by neuronal alpha7beta1 integrin. J Neurosci. 2004 Jan 7;24(1):238-47. doi: 10.1523/JNEUROSCI.4519-03.2004. PMID: 14715956; PMCID: PMC6729556.
• Meiners S, Nur-e-Kamal MS, Mercado ML. Identification of a neurite outgrowth-promoting motif within the alternatively spliced region of human tenascin-C. J Neurosci. 2001 Sep 15;21(18):7215-25. doi: 10.1523/JNEUROSCI.21-18-07215.2001. PMID: 11549732; PMCID: PMC6762977.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.