Recombinant NEDD4 protein (His tag)

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Recombinant Human NEDD4 E3 Ubiquitin-Protein Ligase Fragment (aa 842-1319) is a high-purity protein product encompassing the critical WW4 and HECT domains. This fragment serves as an essential tool for investigating ubiquitin transfer mechanisms, substrate recognition, and the specific functional roles of these domains in cellular signaling, protein degradation, and viral budding pathways.

NEDD4 (Neural precursor cell expressed developmentally down-regulated protein 4) is a HECT-type E3 ubiquitin ligase that directly transfers ubiquitin from E2 conjugating enzymes to substrate proteins, primarily targeting Lys-63 residues. It regulates key cellular processes including receptor internalization and degradation (e.g., VEGFR-2, IGF1R, FGFR1), neuronal development, and autophagosome formation through stabilization of VPS34. NEDD4 also ubiquitinates viral proteins such as Ebola VP40, facilitating viral budding. The protein localizes predominantly to the cytoplasm and nucleus, with membrane association mediated by interactions with partners like GRB10. The provided fragment (aa 842-1319) contains the complete WW4 and HECT domains, enabling focused studies on substrate binding and ubiquitin ligase activity without interference from regulatory regions.