Nal-P-113 peptide

Nal-P-113 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

AKR-Nal-Nal-GYKRKF-Nal, Nal=β-naphthylalanines

12

96.2%

1745.13

Synthetic

Powder

Nal-P-113, a modified version of antimicrobial peptide P-113, its amino sequence is AKR-Nal-Nal-GYKRKF-Nal-NH2. Antimicrobial peptide P-113 showed promising antimicrobial effects against a variety of pathogens. Compared to P-113, Nal-P-113 maintained its effects when exposed to a high salt concentration and therefore it was an ideal candidate for application in complicated matrices including oral cavity, serum and plasma. Nal-P-113 exerts its anti-bactericidal effects in a rat periodontitis model with a significant reduction in tissue inflammation. Nal-P-113 inhibits Porphyromonas gingivalis (P. gingivalis) W83 biofilms formation.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Wang HY, Lin L, Tan LS, Yu HY, Cheng JW, Pan YP. Molecular pathways underlying inhibitory effect of antimicrobial peptide Nal-P-113 on bacteria biofilms formation of Porphyromonas gingivalis W83 by DNA microarray. BMC Microbiol. 2017 Feb 17;17(1):37.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.