NA-CATH peptide

NA-CATH peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KRFKKFFKKLKNSVKKRAKKFFKKPKVIGVTFPF

Lys-Arg-Phe-Lys-Lys-Phe-Phe-Lys-Lys-Leu-Lys-Asn-Ser-Val-Lys-Lys-Arg-Ala-Lys-Lys-Phe-Phe-Lys-Lys-Pro-Lys-Val-Ile-Gly-Val-Thr-Phe-Pro-Phe

34

95.7%

Synthetic

Powder

Discovered in 2008 (see the ref), this peptide is rich in lysines (38%). NA-CATH is predominantly alpha-helical in SDS and TFE (Dean S et al., 2011a). Active against Gram- and Gram+ bacteria (A. actinomycetemcomitans, E. coli, F. novicida, P. aeruginosa, and S. aureus) (Amer L et al., 2010;de Latour F et al., 2010; Dean S et al., 2011a;Dean S et al., 2011b) at concentrations < 1 µM). NA-CATH has low toxicity against host cells (de Latour F et al., 2010). It also has anti-biofilm activity against S. aureus (Dean S et al., 2011a). These studies also showed that small 11-aa peptide variants based on a repeated pattern within NA-CATH (KR(F/A)KKFFKK(L/P)K) have similar antimicrobial and anti-biofilm activity to the parent peptide, but not when proline is present (de Latour F et al., 2010; van Hoek M, 2014). Updated and written by Monique L. van Hoek 5Jan2015; 2/2015. NA-CATH has Antimicrobial activity. The source of NA-CATH is Naja atra.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Zhao H, Gan TX, Liu XD, et al. Identification and characterization of novel reptile cathelicidins from elapid snakes. Peptides. 2008;29(10):1685-91.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.