Myr-tat-CBD3 peptide

Myr-tat-CBD3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Myrisitic Acid-YGRKKRRQRRRARSRLAELRGVPRGL-OH

Myrisitic Acid-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Ala-Arg-Ser-Arg-Leu-Ala-Glu-Leu-Arg-Gly-Val-Pro-Arg-Gly-Leu-OH

26

95.42%

C₁₄₈H₂₆₉N₅₉O₃₃

3403.08

Synthetic

Powder

Myr-tat-CBD3 is the N-terminal myristoylated version of TAT-CBD3 (PN-160), a peptide derived from the axonal collapsin response mediator protein 2 (CRMP-2). The myristoyl group allows insertion of myr-tat-CBD3 into membranes through hydrophobic interactions and myr-tat-CBD3 is membrane tethered, restricts the CaV2.2–CRMP2 interaction, blocks CaV2.2 trafficking, and strongly inhibits Ca2+ influx from primary sensory neurons. Intrathecal injection of myr-tat-CBD3 reduces carrageenan-induced inflammation and reverses paw incision–induced thermal and mechanical hypersensitivity without producing rewarding behaviour.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Brittain et al (2011) Suppression of inflammatory and neuropathic pain by uncoupling CRMP-2 from the presynaptic Ca2+ channel complex. Nature Med. 17(7) 822 www.ncbi.nlm.nih.gov/pmc/articles/PMC3219927/
• Fischer et al (2014) Sustained relief of neuropathic pain by AAV-targeted expression of CBD3 peptide in rat dorsal root ganglion. Gene Ther. 21(1) 44 doi:10.1038/gt.2013.56
• François-Moutal eta al (2015) A membrane-delimited N-myristoylated CRMP2 peptide aptamer inhibits CaV2.2 trafficking and reverses inflammatory and postoperative pain behaviors. Pain 156(7) 1247 doi: 10.1097/j.pain.0000000000000147

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.